Microbiology is a basic and scientific knowledge for Problem-solving in humans from different pathogens. In order to this, a thorough knowledge of microbiology is essential to know exactly which microbes cause diseases. This manual provides a clear knowledge for the isolation of microbes from the different sources, preparation of nutrient supplement, culturing of microorganisms and preservative techniques in laboratories. This manual is very helpful for graduates students, teachers, and researchers. The experiments have been given in chronological order and most of these can be performed with materials available in biology laboratories. For some of the experiments, even alternate procedures have been suggested. The experiments covered in this manual are easy to perform and have been used over the years in our laboratory for imparting practical training regularly to undergraduates and postgraduate students.Experiments were written in a step-wise manner for easy understanding.